The gene for tetracycline resistance is present in the plasmid DNA.Explain the reason for using plasmid DNA?
Eхрlаіn thе reason fοr using plasmid DNA.thаt contain thе gene fοr tetracycline resistance?
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The reason for using plasmid DNA is because in this way you add new qualities to the bacteria and the tetracycline, which is kind of antibiotic, gives the bacteria resistance to substrate with antibiotic. Then you can do an experiment` make 2 gel plates and add to each gel plate antibiotic. antibiotic. Take out from bacterias 2 plasmids and insert to the second plasmid gene for tetracycline. Add one of the plasmids to the first gel plate and the other to the second gel plate. After some days you will see that on the gel plate with the regular plasmid will appear blue clones, however the gel plate with the engineered plasmid (with tetracycline resistance) will have white clones.
I hope u will understand what I meant… check out Xgel and beta gel for the substrate. Hope it helped:) Good Luck!
you can replicate the plasmid dna independently of the chromosonal dna.
you can also take this plasmid and insert it into a bacteria for a whole host of things which i do not feel like elaborating right now